Confocal Microscopes - Medical Research Microscopes, Optical Microscopes

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Optical Microscopes Medical Research Microscopes RTC-7CON Series

Confocal Microscopes

RTC-7CON

Confocal microscope is a high-end product in Radical Optics microscope series. It is designed as an essential microscopy tool for laboratory scientific research, providing powerful and stable imaging capabilities and highly integrated motorization capabilities.
Efficient scanning head, detector and CVT motorized small hole, coupled with powerful optical system, provides fast, stable, high signal-to-noise (S/N) ratio confocal image.
Confocal Microscope provides a variety of motorized parts, including: motorized stage, motorized focusing, motorized nosepiece, motorized fluorescent carousel, motorized condenser and motorized brightness adjustment, operation mode allows physical button operation and software operation, and provides calling commands, which is convenient for users to control and develop by themselves.

High resolution images can be generated with a single click operation, The software will automatically calculate size of the small hole according to objective numerical aperture, exposure value and scanning range, so as to obtain the image with the optimum signal-to noise(S/N) ratio.

At same time, noise reduction algorithm can remove the background noise in real time and improve image quality. Multi-channel images can be collected and synthesized simultaneously, which is convenient for customers to realize real-time observation of multiple stains.

By setting top position, bottom position and movement interval, the Confocal microscope motorized Z axis can realize automatic Z-Stack acquisitionand generate 3D model.

Providing various microscope motorized control interfaces: motorized objective carousel, motorized fluorescent filter unit, motorized condenser turntable.

Motorized stage control and motorized focusing mechanism could locate the Region of Interest (ROI) immediately through the software and record the position so that the user will be able to return to the recorded position quickly.


Part Name	Part Code	Description
Body	RCBD	Rugged, waterproof, ergonomic modular design, multiple camera ports, touch point treated, (rigid and vibration-free) acid resistant texture painted, intensity controller & on/off switch, RL/TL switch, Eco mode*, rubber feet, slot for ana/pol, transmitted diffusion/filters LBD/ND6/ND25, filter holder, collector, scattering filter, automatic re-adjustable illumination intensity etc, inbuilt diaphragm for different contrast methods. In-built power unit for halogen/led, mounted microscopy stand with provision to attach camera in left side/right side port, dual deck facility.
Optical System	RCiOS	Universal infinite fly eye lenses optical system, NIS60 (F200) colour corrected, anti-fungal, anti-bacterial coated (multi-layered hard coated), colour coded objectives, precentered, suitable for bright field, phase / fluorescence / DIC / Emboss /Plas DIC / hoffmann contrast/varel contrast inversion contrast, IMC, DF, ICR, POL, UC-3D imaging with glass and plastic dishes etc.
Eyepiece	RCEP1025	Paired, high eye point WF 10X, FOV-25mm, multilayered coated, high guality, good contrast, abrresion free, aplantic, precentered, diopter adjustment on one/both eyepiece (±5mm)
Illumination	RC5WLED	5W LED cool illumination Life 30,000 hrs, with 4500K (white) (Transmitted) colour temperature, equivalent to 100W halogen, suitable for bright field, phase, IMC, hoffmann, DIC/inversion contrast, Emboss etc. flexible/retroverted ensuring large space, transmitted/epi-fluorescent control knobs located on right side of body-easy to operate, flexible with retroverted (tiltable), buttons for transmitted and reflected easy switch with 2 position filter slider
Viewing Head	RCTST45	Trinocular inclined at 45°, sidentopf head, rotatable through 360°, IPD - from 47-78, diopter adjustment on both eyepiece, top & both side port for camera attachment, splitting ratio100(EP):0(camera) /50:50/0(EP): 100(camera) with 0.7x relay lens placed at side of microscope body, builtin bertrand lens.
Mechnical Stage	RCMC	High precision XYZ motorized stage, with Controller & Joystick Drive System,electric carrier: stroke: 130mm x 110mm (table 325mm x 144mm) Maximum speed: 25 mm/s; resolution: 0.1 μ m. Replicate accuracy: 3 μ m. Mechanical adjustable sample splint, attachable mechanical stage:130(X) × 85(Y) mm, accepts different types of micro-testplate, tissue culture bottles, glass slide holder & culture flask holder, universal holder for holding different types of plate’s petri dishes, cell culture plates, specimen slides of all sizes (6, 12, 24, 96 well).
Nosepiece	RC-6N	Motorized Inward tilted with positive click stop, Precise sextuple revolving on multiple ball bearing, inbuilt DIC slot
Condenser	RABC	Motorized condense, 6 position, long working distance condenser: NA=0.55,WD=26.00mm, with a full set of DIC, PH functions and related accessories for long time culture tracking of live cells.
Focusing	RXF10	Motorized Z-axis focusing mechanism minimum step length set at 10nm adjustment, Focus up 7 down 2, Coarse stroke 2mm per rotation. Fine stroke 0.002mm per rotation, manual and motorized control, minimum step 0.01 µm under motorized control.
Fluorescence Attachment	RCFL6LED	Motorized LED fluorescence lamp house, life upto 20,000hrs., 6 positions Attachment removable turret structure with high position filter including 1 for brightfiled, centre adjustable filed diaphragm and 5 holes filter slot for reflected illumination, with following filter, easy to change (push & click). Motorized Shutter. Fluorescent filter block: cover with UV to visible light, Easy to replace.
Objective	RC10	10X, NA 0.45, W.D. 4.0mm, cover glass thickness 0.17
	RC20	20X, NA 0.75, W.D. 1.1mm,cover glass thickness 0.17
	RC40	40X, NA 0.95, WD=0.25mm-0.17mm
Intermediate	RCI	Manual 1X, 1.5X, Confocal switching
Output Port	RCOP	Splitting Ratio: Left: Eyepiece=100:0; Right: Eyepiece=100:0
DIC Plate	RCDP6	10X，20X，40X, 60x, Plate, Can be Inserted in Nosepiece Slot, motorized shift free with analyzer & polarizer attachment. Slider and modules for objectives
Controller	RCC	Rocking Bar, Controller Box, USB Connection Cable
Laser Unit	RCLU	Laser 405 nm,488 nm,561 nm,633/638nm, 640 nm, Motorized beam path
Detector	RCD	Wavelength: 400-750nm, Detector: 4PMT
Scanner	RCS	Maximum Pixel Size: 4096 Scanning speed: 2fps(512X512), 18fps(256X256), 0.5fps(1024X1024), 0.12fps(2048X2048), 0.03fps (4096X4096)
Scan Mode	RCSM	X-Y, X-Y-Z, X-Y-T
Pinhole	RCPH	Hexagon shape, Continuouslv Variable Transmission(CVT)
Confocal	RCCF	Field number Square Inscribed in a φ18mm Circle
Image bit depth	RCIBD	12 bits
Compatible Microscopes	RCCM	Full Motorized Inverted Microscope
Power Supply	RSMPS620	Integrated SMPS based, wide voltage range, 90V-240V, 50/60Hz Supply
Confocal Scanning & Detection system	Having Built-in Scanner & Detectors Scanning head design: High stability scan head design, connected to the detector via optical fiber for easy disassembly and handling. The confocal scanning head was coupled to the left interface of the microscope body to achieve the highest quality optical path imaging. Scan Head: Maximum pixel size: 8192X8192 Scanning Resolution: 4096X4096 Scan pixels: maximum 4096X4096 up to (8K x 8K) output in the same field of view. Scan Speed: 15 fps @ 512 X 512 Optical scanning zoom：≥1-1000X, in order to accurately scan and locate the details, multistep with ROI scan with 110FPS. Scanning field of view: upto 22mm High sensitivity detector: The high sensitivity detector, having Peltier cooling, quantum efficiency (QE) ≥ 55%, effectively improve the image quality, suitable for high-quality imaging of weak fluorescence. Having 2PMT in standard detector for 2 color simultaneous image acquisition and having capable to perform 4 color sequential imaging. Integrated design: laser, detector and signal acquisition device integrated design, placed in the same cabinet, easy to overall handling and installation. All spectral detectors are built inside the scan head and signals not travel through the fiber for better sensitivity to avoid loss of signal through optical fiber, All the detectors are spectral in nature with choice of selecting the emission bandwidth from both side of the spectrum (400-900nm). In-built Laser power monitor to monitor and compensate against ambient fluctuations for reproducible experiment and quantitative measurement. The spectral resolution is upto 5nm throughout the spectral range of 400-900nm. Supply with Transmitted light detector for capturing bright field and DIC images Scanning mode: lines, faces, rectangle, clip, polygon, free area, circular, Any combination of points/multi-dimensional scanning modes of point, XT, XZ, XY, XZT, XYλ, XYT, XYZ, XYZT, XYλT, XYλZ, XYλZT, along with 3D reconstruction.
Laser Unit	The laser output of all lasers is controlled by the acoustic and optical controller (AOTF), integrated into the scanning head system, and opened with one key, to avoid the risk of color series caused by multiple channels, and to ensure the stability and accuracy of the optical path output; /direct modulation for laser attenuation and switching in synchronization with scanner, approximate lifetime of laser is upto 10000 hrs. Solid-state laser 405nm, Optical fiber export power≥ 20mW; Solid-state laser 488nm, Optical fiber export power≥ 20mW; Solid-state laser 561nm, Optical fiber export power≥ 20mW; Solid-state laser 640nm, Optical fiber export power≥ 20mW;
Software	Image acquisition and system automatic control function, optical road full electric control switch. Complete microscope control, Scan head control and Laser control software Saving of all instrument parameters along with the image for repeatable/reproducible imaging Frame/line/lambda capturing, Z-Stack, Time series imaging capabilities ROI bleach for FRAP experiments, FRED imaging, calcium imaging & deep tissue imaging Co-localization analysis and volume rendering Macro creation HDR Imaging Control the triggering device of laser for microfluidics application Image acquisition & analysis: histogram, distance, intensity measurement (both online & offline) Overtime, over depth and over lambda, colocalization 3D rendering of time lapse imaging, Measurements, Dynamic ROI, EFI/EDF, Background subtraction/correction, bleaching correction, intensity profiling etc. 2D/3D/4D Analysis, deconvolution, Time-lapse Analysis, 3D Volume Render/Orthogonal, Image Stitching, Multi-channel Color Confocal Image Diverse measurement and statistical processing Capable of multi position, mosaic and multi well imaging & stitching for live and fixed imaging High resolution images can be generated with a single click operation, the software will automatically calculate size of the small hole according to objective numerical aperture, exposure value and scanning range, so as to obtain the image with the optimum signal-to noise (S/N) ratio Real-time multi-color image scanning and real-time multi-color image superposition, ratio-display, spectral unmixing, multi-dimensional (x, y, z, t) confocal image acquisition, processing, and reconstruction, multipoint imaging, image stitching. At same time, noise reduction algorithm can remove the background noise in real time and improve image quality. Multi-channel images can be collected and synthesized simultaneously, which is convenient for customers to realize real-time observation of multiple stains. By setting top position, bottom position and movement interval, the motorized Z axis can realize automatic Z-Stack acquisition and generate 3D model. Providing various microscope motorized control interfaces: motorized objective carousel, motorized fluorescent filter unit, motorized condenser turntable. Motorized stage control and motorized focusing mechanism could locate the Region of Interest(ROI) immediately through the software and record the position so that the user will be able to return to the recorded position quickly. Software can perform integrated control for microscopes, cameras, motorized accessories etc. and realize automatic control and image analysis processing. The interface is intuitive and easy to understand, which is convenient for parameter setting and reset.
Active Anti-Vibration Table	A suitable active anti vibration table with active air compressor control with M6 holes to fix the microscope and confocal microscope system AV Table size: 4 Feet X 3 Feet
Compatible Desktop Computer System (Workstation)	Windows 10 Pro 64 bit OS English version CPU: Intel Xeon W-2225( CPU 4.0 core 4) RAM: 64 GB HDD: 1st HP Z turbo G2 512GB, PCIe H.2 SSD. 2nd SATA 2TB Optical Drive: Super Multi drive, upto x 16 speed. LAN: 10/100/1000network interface x 2 Extension Slot: 2 PCI express 3.0 x 16 slots (one slot for Graphics) Graphics：NVIDIAQuadro RTX4000 Monitor：32" monitors Suitable UPS
Accessories	RCC	Cleaning cloth
	RVC	Vinyl/Dust Cover
	RAK	Allen keys
	RF5A	Fuses
	MANUAL	Instruction manual
	WARRANTY	Warranty card Power Cord (country Specific)


Part Name	Part Code	Description
Body	RT7NXFCONBM	Rugged, waterproof, ergonomic modular design, multiple camera ports, touch point treated, (rigid and vibration-free) acid resistant texture painted, intensity controller & on/off switch, rubber feet, slot for ana/pol, filter holder, collector, IR LED base focus drift, scattering filter, switchable mirror to switch light from transmitted to reflected mode, LED indicator (touch panel controller, switching observation settings) automatic controls, enabling switching of optical Components, aperture adjustment & exposure methods with a tap of the touch panel, controller unit with push buttons for all motorized controls, intensity etc, inbuilt diaphragm for different contrast methods, front panel can show status of bright field observation, phase contrast observation, DlC or fluorescence observation, built-in upper port, split ratio 100:0, 0:100, built-in coded* left side port, split ratio 0:100, 50:50, 100:0, there are two layers of light path up and down fluorescent light barrier system can be added, auto adjustment of illumination to the contrast method, auto off function & constant colour temperature.
Front function Display	RTC7NXF-COND	Using capacitive touch screen technology, 7" LCD screen for operation electronic control parts of the body with back light LED guide, can display the objective hole position, component status, observation method and other information, allowing you to intuitively understand the current working status while operation; one- button multi-axis linkage with the component memory function you can switch and adjust at will in the working state to improve work efficiency Microscope retains the traditional coarse and fine adjustment mode cancels the gear mechanism, integrates motorized control technology realizes the integration of manual and automatic, humanized design.
Viewing Head	RT7NXF-CONT45	Tilting 20-45° adjustable binocular head inverted image, interpupillary distance 50-76mm, top & both side port for camera attachment, splitting ratio 100(EP):0(camera)/50 (EP):50 (camera)/0(EP) :100(camera) with 0.7x relay lens placed at side of microscope body, builtin bertrand lens.
Mechnical Stage	RT7NXF-CON	Motorized Programmable Scanning Stage (linear encoded) with Controller & Joystick Drive System: Stepper motor with 2 mm spindle pitch, controlled via XY STEP stage controller Travel Range: 130mm (X) × 100mm (Y), Stage Surface:325 mm × 144 mm, Maximum Speed: 50mm/s Resolution: Sub-micron 0.1µm, Reproducibility: ±1µm, Absolute Accuracy: ±5µm, Motor Cable: Fixed, rearleft exit from stage backside, Mounting: Suitable for standard mounting frames, Controller (XY STEP), Stepper motor control for XY scanning stage, Bidirectional CAN communication with microscope systems, Real-time position display on microscope TFT, Joystick Control with motorized XY mechanical stages, scanning stages (CAN), and XY STEP controller, Direct CAN cable connection to stage or controller, Two selectable speed ranges for coarse and fine control, different types of micro-test plate, petri dish (35mm/60mm) glass slide holder & culture flask holder, universal holder for holding all types of plates, labtek chambers and multi well dishes with multipoint, tile and mosaic imaging software (capable of fast 6D imaging).
Focusing	RXF10i	Motorized Z-axis focusing highprecision mechanism minimum step length set at 25nm adjustment, Focus up 7 down 2, Coarse stroke 2mm per rotation. Fine stroke 0.002mm per rotation, manual and motorized control.
Fluorescence Attachment	RTC-7NXFCONFL	Fluorescence motorized turret 6 position rotating filter holder, std. fluorescence life upto 25000 hrs., 4 high power LED, Band pass fluorescent filters for DAPI, GFP, Cy3 and Cy5, zero-pixel shift technology, noise terminator mechanism, iris diaphragm and aperture diaphragm, with separate power supply & controller, central adjustable, with filter slot and polarizing slot LED controlled through remote/PC software, suitable for FISH application, suitable from UV to Cy7.
	RCFL8LED	Motorized LED fluorescence lamp house, life upto 20,000hrs., 8 positions attachment removable turret structure with high position filter including 1 for brightfiled, centre adjustable filed diaphragm and 7 hole filter slot for reflected illumination, with following filter, easy to change (push & click), with inbuilt shutter. fluorescent filter block, suitable from UV to Cy7.
Objective	RC20i	20X, NA 0.80, W.D. 1.1mm, air with correction collar
	RC60	60X, NA 1.40, W.D. 0.14mm, oil, spring loaded, with correction collar
	RC100	100X, N.A. 1.45, W.D. 0.13mm, oil immersed, spring loaded with correction collar
Spinning disk confocal imaging unit	Spinning disk scanning confocal system with high sensitive CCD or CMOS detectors. Detection unit work in epi-fluorescence and confocal. real time confocality with a by pass mode. System have Excitation range of 390-750nm and emission range of 430nm-850nm, capable of capturing 22mm field of view. Minimum XY resolution of 230nmand Z resolution of 600 nm.

Confocal Microscopes RTC-7CON Series

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